By Waldo E. Cohn (auth.), Seymour Rothchild (eds.)

ISBN-10: 1468475320

ISBN-13: 9781468475326

ISBN-10: 1468475347

ISBN-13: 9781468475340

The 11th Symposium on Advances in Tracer process­ ology used to be held in Boston on October 13-14, 1966. The sym­ posium, which was once backed by way of the hot England Nuclear company, honored the 10th anniversary of the company. The Symposia on Advances in Tracer technique, that have been held frequently considering 1957, collect learn staff in a number of disciplines who percentage an curiosity in radioactive tracers. The 4 volumes, this one representing the list of the 11th Symposium, offer a compact and on hand resource of information-much of which was once often scattered through the clinical literature-of inter­ est to biochemists, pharmacologists, endocrinologists, and analysts for whom tracers became an essential software. The editor is mainly thankful to Dr. Konrad Bloch, Harvard collage, and Dr. Waldo E. Cohn, Oak Ridge nationwide Laboratory, for chairing the periods on the 11th Sym­ posium. The cooperation of the audio system is deeply liked. moreover, a different bouquet to my secretary, Mrs. Edith Thompson, for her beneficiant suggestions in arranging a few of the symposia and the ebook of the papers. S. R.

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Nucleonics 20:86 (1962). 11. , Radiochirn. Acta 1:174 (1963). 12. , and Sollich, W. , J. Catalysis 2:350 (1963). 13. Garnett, J. , and Sollich-Baurngartner, W. , J. Phys. Chern. 69:1850 (1965). 14. Garnett, J. L.. and Sollich, W. , Australian J. Chern. 18:1003 (1965). 15. , J. Phys. Chern. 68:3887 (1964). 16. , Australian J. Chern. 18:993 (1965). 17. , Australian J. Chern. 19:2299 (1966). 18. Garnett, J. , and Sollich, W. , J. Catalysis 2:339 (1963). 19. , Australian J. Chern. 14:441 (1961). 20.

Iution) Dowex SO, No + Eluent: sodium citrate buffer, pH 5. 35 M Mixture of 8 AI. Iution) Fig. 1. l. 48 Labeled Compounds necessary background for a discussion of the design of criteria of purity for these compounds. The first and foremost consideration in selecting paper chromatographic systems for the determination of purity is their capability of detecting cross contamination between an amino acid and those that are eluted immediately before and after and between any other contaminants that are likely to occur.

SUMMARY A major parameter in the valid application of tracer methodology employing isotopically labeled compounds is the purity of the tracer compound employed. The ultimate requirement of purity rests upon the design of the experiment, which must allow for the nature of the impurities that might be present and the levels that are permissible and yet yield meaningful data from which valid conclusions may be drawn. As the situation exists today, where many if not most investigators no longer prepare the labeled compounds they employ, but obtain them from commercial laboratories, the criteria of purity that are used by the manufacturer cannot always satisfy each and every investigator's requirements.

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Advances in Tracer Methodology: Volume 4: A collection of papers presented at the Eleventh Annual Symposium on Tracer Methodology by Waldo E. Cohn (auth.), Seymour Rothchild (eds.)


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